The team is composed of approximately 28 players. Preseason starts February 15, with players practicing in the spacious indoor field house until late March when the team takes its annual trip to Florida or Arizona to compete in 10 to 12 games over spring break.
Colby competes in the Eastern Division of the New England Small College Athletic Conference (NESCAC) and plays three-game series with Bates, Bowdoin, Trinity, and Tufts. The top two teams from the East Division play the top two teams from the West Division for an automatic berth in the NCAA tournament. The Mules also play non-conference games against in-state rivals such as Southern Maine. League games are played on Fridays and Saturdays, and weekday games are in-state to ensure as little missed class time as possible. Colby has had many outstanding players recognized by the league with NESCAC All-Conference honors in recent years. Mike Mastrocola '12, Robb Arndt '12, and Jason Buco '15 all earned All-NESCAC honors in 2012. Richard Newton '11 earned NESCAC second team honors and was on the All-New England squad. Craig Cooper '09 earned all-conference second team honors in 2009, Tom Salemy earned first team honors in 2007 and Andy Carr was selected to the first team in 2006. Nick Spillane '08 was named league Rookie of the Year and earned second-team honors in 2005. Second baseman Andrew Tripp '01, outfielder Jon Lord '02, and catcher Eric Roy '04 all were two-time selections, and Tripp and Lord each earned All-New England honors. Kevin Brunelle '02 was All-NESCAC, while Kristopher Keelty '99 went on to pitch professionally for two seasons for the independent league Adirondack Lumberjacks. Former pitching ace Galen Carr '97 is currently scouting for the Boston Red Sox.
Many of Colby's current players are active with summer baseball teams, and part of the team includes two-sport athletes.