BC 367- Biochemistry of the Cell I

Instructors:

Julie T. Millard, Dorros Professor of Life Sciences
Lecture and Laboratory B
Keyes 304, x5757

Brenda L. Fekete, Senior Teaching Associate
Laboratories A and C
Keyes 310, x5768


The molecules that we will discuss this semester tend to be quite large and complex. Shown here is hemoglobin, the vertebrate oxygen-carrying protein of the bloodstream.


Course Objectives
We will focus on the biological molecules, chemical reactions, and regulatory processes that are essential to the functioning of the cell. Topics include the structures and functions of the major classes of biological molecules (proteins, carbohydrates, nucleic acids, and lipids) and enzyme kinetics and mechanisms.

Attendance Policy
Attendance at every class meeting and laboratory session is mandatory. Unexcused absences from class will result in a reduction of your grade (at our discretion). Even one unexcused absence from laboratory will result in your dismissal from the course. Please keep in mind that only your instructors can determine what constitutes an excused absence for this course.


Course Materials:

Syllabus

Sample Exam 1

Multiple Choice Review for Exam 1

Solutions to Your First Exam

Sample Exam 2

Multiple Choice Review for Exam 2

Solutions to Your Second Exam

DNA on Trial Powerpoint

Chemistry and the Genetic Revolution

Useful Links:

QuickPDB

PubMed

Carbonic Anhydrase Structure at the PDB

Primer3

Biochemistry - Author Information

ExPASy (The Expert Protein Analysis System proteomics server of the Swiss Institute of Bioinformatics)

Online Mendelian Inheritance in Man

Protein Data Bank


Laboratory Materials:

Syllabus

Experiment 1: Identification of an Unknown Amino Acid

Experiment 2: Comparison of Protein Assays

Ocean Optics Instructions for Experiment 2

Experiment 3: Purification and Characterization of the Enzyme Lysozyme

Ocean Optics Instructions for Experiments 3 and 4

Experiment 4A: Kinetic Properties of Acid Phosphatase

Experiment 4B: Acid Phosphatase Computer Modeling

Experiment 5: Forensic Analysis of Canine DNA