We have been very fortunate to receive four National Science Foundation Grants during the
2001-2002 years. On this page we will summarize the curriculum materials that we have
prepared under these grants.
Biochemical, Organic, Physical, Analytical, and Inorganic Mass Spectrometry
The proposal: Project Description,
References,
Budget Justification,
and Summary.
The lab experiment that we developed for General Chemistry:
Synthesis of Metal Acetylacetonates: Preparation of Tris(2,4-pentanedionato)chromium(III)
[Adapted from Z. Szafran; R.M. Pike; M.M. Singh; Microscale Inorganic Chemistry, Wiley:
New York, 1991.]
and the characterization
Electrospray Mass Spectrometry of Tris-acetylacetonatochromium(III)
or these two
combined:Synthesis and MS Characterization of Tris-acetylacetonatochromium(III)
.
We developed a Web applet to help find the stoichiometry of a transition metal complex
from its mass. Students particularly liked this applet:
Complex Finder.
This applet should be very useful for general research projects, so it is more than a
teaching tool. A companion Web applet allows students to predict the isotope peak
profile for a given formula:
Isotope Cluster.
The lab experiment that we developed for the Organic course:
Solid Phase Synthesis
and Mass Spectrometric Analysis of a FMOC-Dipeptide
[adapted from G. A. Truran, K. S. Aiken, T. R. Fleming, P. J. Webb, and
J. H. Markgraf, J. Chem. Ed., 2002, 79(1), 85-86; and
A. Taralp, C. H. Turkseven, A. O. Cakmak, and O. Cengel, J. Chem. Ed.,
2002, 79(1), 87-89.]
and the characterization
Electrospray Mass Spectrometry of FMOC-Dipeptides
We developed a Web applet to help find the m/z of a short peptide, which students
found very useful:
Peptide Mass Finder.
This applet is similar to those at UCSF-Protein Prospector, except that protecting
groups are allowed at the terminus of the peptide and a variety of post-translational
modifications are included.
Other Web Calculation Applets
Our students have found "helper" applets very useful, especially while they are still
new to mass specrometry. Peptide and protein based calculations are very
common, but nucleotide based applets are not as common. We have also developed
an applet that searches for the sequence that matches a given m/z for short
oligonucleotides:
Pentanucleotide
Mass Finder
Tutorials and Instructions
Our
Instrumentation Web page has tutorials
and instructions for many of our major instruments. The documents
developed under this grant are also listed below:
Assessment
We developed two Web based surveys for this grant:
General Chemistry LC/MS and
Organic Chemistry LC/MS. These surveys are easy to modify for other
uses. The
cgi script that runs the survey is also available if you
would like to use it.
This material is based upon work supported by the National Science Foundation
under Grant No. 0088307.
Any opinions, findings, and conclusions or recommendations expressed in this
material are those of the author(s) and do not necessarily reflect the views of
the National Science Foundation.
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